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Nano-bio Interface

Membrane Curvature at the Nano-bio interface

The goal of this project is to understand how membrane curvature regulates biochemical signals at the nano-bio interface. Here, the nano-bio interface refers to the physical interface between the cell membrane and nanoscale surface topography. We use nanofabrication to precisely engineer nanostructures that regulate the location, degree, and sign (positive or negative) of membrane curvatures in live cells. We found that membrane curvatures at the nano-bio interface induce significant changes of intracellular processes including endocytosis, actin dynamics, and mechanotransduction.

Selected Publications

- Li X, Klausen LH, Zhang W, Zeinab J, Tsai CT, Li TL, Cui B.
Nanoscale Surface topography reduces focal adhesions and cell stiffness by enhancing integrin endocytosis
Nano Letters, (2021). [Link]

- Roy AR, Zhang W, Jahed Z, Tsai CT, Cui B, Moerner WE.
Exploring cell surface-nanopillar interactions with 3D super-resolution microscopy
bioRxiv, (2021). [Link]

- Zhang, W., Yang, Y., Cui, B.
New perspectives on the roles of nanoscale surface topography in modulating intracellular signaling.
Current opinion in solid state & materials science, 25, 100873 (2021). [Link]

- Li, L., Guo, Q., Lou, H., Liang, J., Yang, Y., Xing, X., Li, H., Han, J., Shen, S., Li, H., Ye, H., Di Wu, H., Cui, B., Wang, S.
Nanobar Array Assay Revealed Complementary Roles of BIN1 Splice Isoforms in Cardiac T-Tubule Morphogenesis.
Nano Letters 20, 6387-6395 (2020). [Link]

- De Martino S, Zhang W, Klausen L, Lou HY, Li X, Alfonso FA, Cavalli S, Netti PA, Santoro F, Cui B.
Dynamic Manipulation of Cell Membrane Curvature by Light-Driven Reshaping of Azopolymer
Nano Letters 20, 577-584 (2020). [Link]

- Lou HY, Zhao W, Li X, Duan L, Powers A, Akamatsu M, Santoro F, McGuire AF, Cui Y, Drubin DG, Cui B.
Membrane curvature underlies actin reorganization in response to nanoscale surface topography
Proc. Natl. Acad. Sci. USA 116, 23143-23151 (2019). [Link]

- Li X, Matino L, Zhang W, Klausen L, McGuire AF, Lubrano C, Zhao W, Santoro F, Cui B,
A nanostructure platform for live-cell manipulation of membrane curvature
Nature Protocols 14, 1772-1802 (2019). [Link]

- Lou HY, Zhao W, Zeng Y, Cui B,
The Role of Membrane Curvature in Nanoscale Topography-Induced Intracellular Signaling
Accounts of Chemical Research 51, 1046-1053 (2018). [Link]

- Santoro F, Zhao W, Joubert LM, Duan L, Schnitker J, van de Burgt Y, Lou HY, Liu B, Salleo A, Cui L, Cui Y, Cui B,
Revealing The Cell-Material Interface With Nanometer Resolution By Focused Ion Beam/Scanning Electron Microscopy
ACS Nano 11, 8320-8328 (2017). [Link]

- Zhao W, Hanson L, Lou HY, Akamatsu M, Chowdary PD, Santoro F, Marks JR, Grassart A, Drubin DG, Cui Y, Cui B,
Nanoscale manipulation of membrane curvature for probing endocytosis in live cells
Nature Nanotechnology 12, 750-756 (2017). [Link]

- Hanson L, Zhao W, Lou HY, Lin ZL, Lee SW, Chowdary PD, Cui Y, Cui B,
Vertical nanopillars for in situ probing of nuclear mechanics in adherent cells,
Nature Nanotechnology, 10, 554-562 (2015). [Link]